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macro-prep hq anionexchange support  (Bio-Rad)


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    Structured Review

    Bio-Rad macro-prep hq anionexchange support
    Macro Prep Hq Anionexchange Support, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anionexchange+supports/macro+prep+hq+anionexchange+support/pm18390849-89-6-10
    Average 90 stars, based on 1 article reviews
    macro-prep hq anionexchange support - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Wound healing involves induction of cyclooxygenase-2 expression in rat skin.
    Article Snippet: .. Then, 3-1-propanesulfonate (CHAPS; Sigma Chemical, Steinheim, Germany) was added to yield a final CHAPS concentration of 1% (wt/vol), and the mixture was stirred for 2 hours at 4° C. After centrifugation at 15,000 rpm for 20 minutes at 4° C, the supernatant was concentrated to 50% of the initial volume using an ultrafiltration unit (Advantec, Tokyo, Japan) and loaded onto anionexchange supports (Macro-prep High Q Supports; Bio-Rad Laboratories, Hercules, California) equilibrated with 20 mM Tris-HCl (pH 8.0) and 0.4% CHAPS. .. Fractions eluted at 1.0 M NaCl were normalized using a DC protein assay kit (Bio-Rad).

    Centrifugation:

    Article Title: Wound healing involves induction of cyclooxygenase-2 expression in rat skin.
    Article Snippet: .. Then, 3-1-propanesulfonate (CHAPS; Sigma Chemical, Steinheim, Germany) was added to yield a final CHAPS concentration of 1% (wt/vol), and the mixture was stirred for 2 hours at 4° C. After centrifugation at 15,000 rpm for 20 minutes at 4° C, the supernatant was concentrated to 50% of the initial volume using an ultrafiltration unit (Advantec, Tokyo, Japan) and loaded onto anionexchange supports (Macro-prep High Q Supports; Bio-Rad Laboratories, Hercules, California) equilibrated with 20 mM Tris-HCl (pH 8.0) and 0.4% CHAPS. .. Fractions eluted at 1.0 M NaCl were normalized using a DC protein assay kit (Bio-Rad).



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